A credible method was developed for the simultaneous determination of eleven steroid hormone residues in animal muscle tissues and eggs based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The eleven steroid hormones were testosterone, methyltestosterone, trenbolone, boldenone, nandrolone, methandienone, stanozolol, progesterone, nadrolone propionate, testosterone propionate and nadrolone phenylpropionate. The samples were extracted with tert-butyl methyl ether at alkaline pH and then cleaned up by freezing-lipid filtration. All these drugs can be assayed in 10 min by UPLC-MS/MS using electrospray ionization in positive ion mode and multiple reaction monitoring mode. The limits of detection were microg/kg for testosterone, methyltestosterone, boldenone, methandienone and stanozolol, and microg/kg for trenbolone nandrolone, progesterone, testosterone propionate and nadrolone phenylpropionate. Overall recoveries of testosterone, methyltestosterone, boldenone, methandienone and stanozolol were % - 105% from pork, beef, mutton and chicken muscle tissues, and eggs fortified at the 1, 2 and 10 microg/kg levels, and the relative standard deviations (RSDs) were % - 15%. The recoveries of trenbolone nandrolone, progesterone, testosterone propionate and nadrolone phenylpropionate were higher than %, and the RSDs were lower than 16%. The matrix calibration curve for each drug was linear (r > ) from 1 to 100 microg/L. The established method is simple, rapid, sensitive and specific, and is appropriate for the identification and quantification of anabolic androgenic steroids in animal muscle tissues and eggs.
Laws and Penalties: Concerns over growing illegal AAS abuse by teenagers, and many of the just discussed long-term effects, led Congress in 1991 to place the whole AAS class of drugs into Schedule III of the Controlled Substances Act (CSA). Under this legislation, AAS are defined as any drug or hormonal substance, chemically and pharmacologically related to T (other than estrogens, progestins, and corticosteroids) that promotes muscle growth. The possession or sale of AAS without a valid prescription is illegal. Since 1991, simple possession of illegally obtained AAS carry a maximum penalty of one year in prison and a minimum $1,000 fine if this is an individual’s first drug offense. The maximum penalty for trafficking (selling or possessing enough to be suspected of selling) is five years in prison and a fine of $250,000 if this is the individual’s first felony drug offense. If this is the second felony drug offense, the maximum period of imprisonment and the maximum fine both double. While the above listed penalties are for federal offenses, individual states have also implemented fines and penalties for illegal use of AAS. State executive offices have also recognized the seriousness of AAS abuse and other drugs of abuse in schools. For example, the State of Virginia enacted a law that will allow student drug testing as a legitimate school drug prevention program (48, 49).
Experienced users can opt for slightly higher levels of dosage. However, like other steroids, Dianabol’s effects too plateau off beyond a certain dosage, and majority of experienced users keep themselves well below the dose range of 70-80 mg per day. However, they can augment Dianabol’s anabolic properties with a stack of other steroids used for mass building. Deca-Durabolin and Testosterone Enanthate are two steroids that can complement Dianabol . Consuming a diet rich in calories and essential nutrients is also important during Dianabol treatment. This anabolic/androgenic steroid has been the favorite of bodybuilders for several decades now and when used within recommended limits, and with a well-balanced diet can result in remarkable muscle growth.