Thin-layer chromatographic analysis of steroids a review

“The twist in the story is that we thought that the distance between the lights in the string was the most important. It is important, but what is more important is the way that multiple strings and their lights are carefully arranged. It turns out that the processing solvent we used acts to arrange and regulate the architecture, so the azobenzene molecules attached to the polymer are arranged very neatly and compactly. It basically acts to ensure that there can be maximum packing density.” says Venkataraman. Details appear in the current issue of Scientific Reports .

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Since different compounds will travel a different distance in the stationary phase, chromatography can be used to isolate components of a mixture for further analysis. The separated compounds each occupying a specific area on the plate, they can be scraped off (along with the stationary phase particles) and dissolved into an appropriate solvent. As an example, in the chromatography of an extract of green plant material (for example spinach ) shown in 7 stages of development, Carotene elutes quickly and is only visible until step 2. Chlorophyll A and B are halfway in the final step and lutein the first compound staining yellow. Once the chromatography is over, the carotene can be removed from the plate, extracted into a solvent and placed into a spectrophotometer to determine its spectrum. The quantities extracted are small and a technique such as column chromatography is preferred to separate larger amounts.

2. Semidestructive visualization . Set up a developing tank (150-ml beaker) but leave out the filter paper and any solvent. Just a beaker with a cover. Add a few crystals of iodine. Iodine vapors will be absorbed onto most spots of compound, coloring them. Removing the plate from the chamber causes the iodine to evaporate from the plate, and the spots will slowly disappear. Not all spots may be visible. So if there’s nothing there, that doesn’t mean nothing’s there. The iodine might have reacted with some spots, changing their composition. Hence the name semidestructive visualization.

Thin-layer chromatographic analysis of steroids a review

thin-layer chromatographic analysis of steroids a review

2. Semidestructive visualization . Set up a developing tank (150-ml beaker) but leave out the filter paper and any solvent. Just a beaker with a cover. Add a few crystals of iodine. Iodine vapors will be absorbed onto most spots of compound, coloring them. Removing the plate from the chamber causes the iodine to evaporate from the plate, and the spots will slowly disappear. Not all spots may be visible. So if there’s nothing there, that doesn’t mean nothing’s there. The iodine might have reacted with some spots, changing their composition. Hence the name semidestructive visualization.

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